Eco-friendly Green Synthesis of Silver Nanoparticles (AgNPs) via Aqueous Extraction from Annona Squamosa Leaves and Its Genetic Effects on the MCF-7 Cell Line

Document Type : Original Article

Authors

1 Molecular Regeneration Laboratory, Department of Reproductive Health, National Research Centre.

2 Department of Applied Radiation Biology Nuclear Research Centre, Atomic Energy Authority.

3 Agricultural Biochemistry Department, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt Postal Code: 11823.

Abstract

Nanotechnology treatments are among the numerous unconventional cancer medicines that have emerged in recent years. Even with its positive results, additional research and study are required. This work included the green synthesis of silver nanoparticles (AgNPs) utilizing an aqueous extract of Annona Squamosa L. The aqueous extract was analyzed qualitatively and quantitatively for phytochemical content. The morphological features, optical characteristics, and surface properties of synthesized AgNPs were examined using transmission electron microscopy (TEM), ultraviolet-visible (UV-Vis) absorption spectroscopy, transform infrared spectroscopy (FTIR) and Dynamic Light Scattering (DLS). The analysis revealed phenols (8.70 mg GAE/g), and flavonoids (7.50 mg/g). The plant extract demonstrated antioxidant activity, attributed to its high flavonoid and phenolic content, suggesting its potential source demonstrated that the bio-synthesized AgNPs exhibited a remarkable level of homogeneity concerning their surface morphology. The mean diameter of the particles was around ~16.7 nm to ~37.0 nm. A. squamosa extract and AgNPs showed low toxicity and effective treatment trial for cancer with a significant decrease in cell viability with a percentage (58.2%) and (79.57%) respectively. In conclusion, the present work introduces a straightforward and economically viable approach for synthesizing AgNPs using environmentally friendly processes, intending to use them as a promising treatment for breast cancer of MCF-7 cell line

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