In Vitro Calli Developing and Characterizing from Sweet Potato (Ipomoea batatas) to Maximize Secondary Metabolites Production

Document Type : Original Article

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Biotechnology Department, Faculty of Agriculture, Al-Azhar University, Nasr City, Cairo 11651, Egypt

Abstract

Sweet potato (Ipomoea batatas) is considered as an important vegetable according to its extreme nutritive value. It’s also a medicinal plant with high source of vitamins, minerals, anti-inflammatory, anticancer and antidiabetic activity. Biotechnology approaches are used to maximize the added value of sweet potato which is needed to fill the gap of world hunger crisis. plant tissue culture technology is a perpetual tool that provides deep understanding of morphogenesis, biochemistry, and molecular physiology in organized and disorganized plant culture. The objective of this study is to find out the effective recommended media composition for callus initiation and induction from leaf explant of sweet potato. Calli are achieved by growing explants on eight treatments which were prepared from MS nutrient medium with different concentrations of plant growth regulators (PGR). Previous treatments were evaluated using cell viability, cell growth and callus type as parameters after nine weeks. Results showed that (2,4-D 2 mg/L to BA 2 mg/L) or (NAA 2 mg/L to BA 2 mg/L) were the best auxin and cytokinin concentrations. Also, results showed that callus initiated from (2,4-D and BA) cultures was more friable than (NAA and BA) cultures. Finally, disorganized cultures (calli) were essential step for continuous cell suspension to produce secondary metabolites.

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