The Influence of Oocyte Diameter and Culture Media Type on in vitro Nuclear Maturation in Camel

Document Type : Original Article

Authors

1 Animal Production Department, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.

2 Zoology Department, Faculty of Science, Benghazi University, Libya.

3 Animal Production Department, Faculty of Agriculture, Mansoura University, Egypt.

4 Biotechnology Department, Animal Production Research Institute, Agriculture Research Center, Egypt.

Abstract

This study aimed to determine the influence of oocyte diameter and culture medium (tissue culture medium (TCM-199) and minimal essential medium (MEM)) on in vitro nuclear maturation in camel (Camelus dromedarius). The recovered oocytes (523 oocytes) were classified into three diameter categories: including ≤120, 120-150, and ≥150 µm. The results revealed that Oocytes matured in TCM-199 had a significantly (p<0.05) higher percentage of oocytes reaching the metaphase II (MII) (46.34%) than oocytes matured in MEM medium (38.63%). The percentage of oocytes arrested at MII was significantly (p<0.05) higher for oocytes with a diameter of ≥150µm (53.25%), moderate for 120-150µm (43.36%) and lowest for ≤120µm (24.16%) oocytes, regardless of the type of maturation medium. The interaction between culture medium and oocyte diameter was insignificant, except for interaction between TCM-199 medium and ≥ 150 μm of oocyte diameter, reflecting a higher percentage of oocytes arrested at MII (58.88%). In conclusion, oocytes with a diameter of ≥ 150 µm matured in TCM-199 medium have the potential to improve the in vitro maturation of camel oocytes.

Keywords

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Main Subjects

Al-Azhar Journal of Agricultural Research
Volume 48, Issue 2
December 2023
Pages 155-162

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