Chemical composition and secondary metabolites of Egyptian faba bean (Vicia faba L.) effected by germination and soaking

Document Type : Original Article

Authors

1 Department of Biochemistry, Faculty of Agriculture, Al-Azhar University, Cairo. Egypt.

2 Department of Agronomy, Faculty of Agriculture, Al-Azhar University, Assuit, Egypt.

Abstract

Abstract
Two Egyptian faba bean v (Sakha- were
soaking in distilled water for 24, 48 and 78 h to dry seeds, and
germination. For both varieties, the
moisture, lipid, ash, nitrogen, protein and carbohydrates contents were determined
. Secondary metabolites concentrations were estimated, Total
phenolic Total flavonoids , Total alkaloids, Phytic acid and
Tannins. A proximate composition of faba bean seed
varieties (Sakha-1 and Giza-843) showed that, Giza-843 seeds contain
higher level of moisture, ash content, crude fiber and total carbohydrates
(7.53, 3.75, 6.81 and 50.61 %, respectively). The Chemical compositions
of Sakha-1 seeds were 1.65, 5.03 and 31.44% for crude lipid, total
nitrogen and crude protein respectively. Results showed that the variety
Sakha-1 was higher than Giza-843 variety in Total phenolic
(TPCs), Total alkaloids (TAs) and Phytic acid. Total flavonoids (TFs) in
the Giza-843 seed (81.34 mg/100g) were higher than those found in the
Sakha-1 seeds (80.23mg/100g. Also, Tannins were higher in Giza-843
seed (653.25 mg/100g) than in Sakha-1 seeds (635.59mg/100g). Soaking
process of variety (Sakha-1 and Giza-843) in distilled water for (24,48
and 72 hours) significantly lowered total ash content and crude fiber
compared with control but , it was increased moisture, crude lipid, crude
protein and total carbohydrates compared with control. Data of soaked
seeds in water showed a slight reduction in TPCs, TFs and Tannins
contents, while the results of Total alkaloids and Phytic acid were
increased compared with control. Germination processes caused a
decrease of moisture, crude lipid and total carbohydrates.

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