Study of phytochemical analysis and antimicrobial activity of ethanolic extract of Nigella sativa L. and Matricaria chamomilla

Mekky, Alsayed E.; Elkhawas, Ibrahim A.; Hamed, Abdou M.; Elhaw, Mohamed H.; Elsousy, Eman M.

doi:10.21608/ajar.2022.145237.1067

Study of phytochemical analysis and antimicrobial activity of ethanolic extract of Nigella sativa L. and Matricaria chamomilla

Document Type : Original Article

Authors

¹ Botany and Microbiology Department Faculty of Science Al-Azhar University

² Botany and Microbiology Department, Faculty of Science, Al-Azhar Universit

³ Botany and Microbiology Department, Al-Azhar University, Nasr City 11884, Cairo, Egypt

⁴ Botany and Microbiology Department, Faculty of Science, Al-Azhar University

⁵ Consultant, PhD microbiology, American board clinical pathology

10.21608/ajar.2022.145237.1067

Abstract

Nigella sativa L. and Matricaria chamomilla are widely used as a traditional medicine since ancient times in which some of them substances are believed to contain anti-oxidants and anti-inflammatory, antimicrobial and antitumor compounds. Objective: to assess the quantitative assay for Alkaloids, Tannins, Phenolic acids, Saponins and Flavonoids in Nigella and Chamomile extracts with evaluation of their antimicrobial activity. Results: the quantitative assay for Nigella and Chamomile extracts were total flavonoids (295.21±0.51 and 263.12±1.05 mg/gm), total phenolic acids (388.60±1.02 and 302.10±0.88 mg/gm), total Tannins (1.23±0.11 and 1.33±0.13%), total Saponins (1.71±0.10 and 1.66±0.16%) and Total Alkaloids (2.01±0.20 and 1.07±0.14 %) respectively. Based on the observed results, Nigella and Chamomile extracts were an active anti-microbial substances. Diameters of the inhibition zones of Nigella and Chamomile extracts were about (17 and 15 – 14 and 18 – 8 and 8 – 12 and 10 – 15 and 13 – 16 and 14 mm) against S. aureus, S. haemolyticus, E. coli, K. pneumoniae, C. albicans and C. glabrata respectively. The (MICs) of Nigella against the bacterial and candida strains ranged from 250 μg/ml to 500 μg/ml and (MLCs) ranged from 500 μg/ml to 1000 μg/ml. where The (MICs) values of Chamomile against the bacterial and candida strains ranged from 500 μg/ml to 1000 μg/ml and minimum lethal concentrations (MLCs) ranged from 1000 μg/ml to 2000 μg/ml. Finally, this study demonstrates that Nigella sativa and Chamomile efficacy as an alternative therapeutic agents in treatment of many microbial diseases to provide a comprehensive guide for pathologists.

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